1339 Casein kinase 1 inhibition in keratinocytes enhances the migration of melanocyte precursors in the hair follicle via KitL/c-Kit signaling pathway

Melanocyte regeneration in epidermis (e.g., vitiligo) and matrix of hair follicle graying hair) is quite dependent on the migration melanocyte precursors located follicle. We have demonstrated that casein kinase (CK) 1 ablation keratinocytes increases eumelanin production epidermal numbers. Here we further evaluate effect CK1 inhibition first established Fusion Red-labelled-melanocyte reporter mice C57BL6 (WT) melanocortin receptor mutated (Mc1r549del), then topically applied inhibitor (CKI) back skin for 4 weeks. Skins were harvested at different time point tissue analysis. Confocal microscopy showed increased numbers precursor secondary germ outer root sheath first, later. RT-PCR Western blotting up-regulation p53/KitL/c-Kit/MITF/tyrosinase signaling pathway both mice. Eumelanin shaft Mc1r549delmice. CKI treatment human keratinocyte culture enhanced KitL production; culture, cell migration. In conclusion, topical induces epithelium follicle, which stimulating to causing shaft. Thus, provides a UV sparing strategy regenerate vitiligo or via activation KitL/c-Kit pathway; not indispensable Mc1r.